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Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation - a 2 minute Preview of the Experimental Protocol
Emily A. Brown, Steven C. Neier, Claudia Neuhauser, Adam G. Schrum, Stephen E.P. Smith
Seattle Children's Research Institute, Center for Integrative Brain Research; University of Washington, Graduate Program in Neuroscience; Harvard Medical School, Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine; Broad Institute of Harvard and MIT,; University of Houston, Department of Mathematics; University of Missouri, Department of Molecular Microbiology and Immunology, School of Medicine; University of Missouri, Department of Surgery, School of Medicine; University of Missouri, Department Bioengineering, College of Engineering; University of Washington, Department of Pediatrics;
Quantitative Multiplex Immunoprecipitation (QMI) uses flow cytometry for sensitive detection of differences in the abundance of targeted protein-protein interactions between two samples. QMI can be performed using a small amount of biomaterial, does not require genetically engineered tags, and can be adapted for any previously defined protein interaction network.
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