Seminar Abstract:
Join us for an overview of @OxfordNanoporeTechnologies' sequencing tools, applications, and recent advancements.

Chemistry Overview – R10.4.1 flow cells with the latest Kit14 chemistry deliver high-accuracy, high-output sequencing data, achieving 99.6% single molecule raw-read simplex accuracy and 99.92% duplex accuracy. The newer chemistry offers tunable run conditions, providing more flexibility between accuracy and output.

Methylation analysis – Study methylation without bisulfite conversion. Bioinformatics tools, such as Remora, provide a comprehensive workflow for characterizing methylation.

Short Fragment Mode – Sequence fragments as short as 20bp and study the nucleosome of cell-free DNA.

Adaptive Sampling – Enrich or deplete regions of interest at the software level—no need to enrich for your target upstream of sequencing.

Application Focus – Single-cell transcriptomics using 10X libraries; Inexpensive rapid microbial whole-genome; Full-length plasmid sequencing without tiling; Immunogenetics; Native RNA sequencing to explore RNA base modifications; Bulk RNA-seq for analyzing gene expression, full-length isoform, and alternative splicing.

Chapters:
0:00 – Welcome and Introduction to the Oxford Nanopore Team
2:08 – Nanopore DNA/RNA Sequencing
8:13 – Methylation, WGS & Targeted Sequencing
21:40 – RNA Research
28:09 – AAV/Plasmids
39:36 – Microbial & Metagenomics
47:47 – Summary
50:22 – Q&A

To learn more about Oxford Nanopore:
Flongle – [ Ссылка ]
MinION – [ Ссылка ]
GridION – [ Ссылка ]
PromethION 2 – [ Ссылка ]

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