This is part 1, session 1 of the Cryopreservation for Regenerative Medicine workshop which was held on May 5, 2021, The event was jointly hosted by the Canadian Stem Cell Network and the Society for Low Temperature Biology. This cryopreservation educational workshop is designed to inform participants on the principles, best practices and latest advances in cryopreservation.
Part 1, Session 1: Fundamental Principles of Cryopreservation
Session Chair: Dr. Christiani Amorim, Université Catholique de Louvain, Belgium, Chair SLTB
Fundamental principles for cryopreservation of animal cells
Dr. Ram Devireddy, Louisiana State University, USA
Learning objectives:
• The physical changes occurring in cooling cells during cryopreservation and vitrification
• The role of cryoprotectants and the importance of osmosis in slow cooling methods
• Impact of morphological and physiological variation between different cell types
Cryoprotectants - Balancing Toxicity and Efficacy
Dr. Gloria Elliott, University of North Carolina at Charlotte, USA
Learning objectives:
• The key types of cryoprotectant and their mode of action.
• Appreciate the diverse biological effects of DMSO, the balance of toxicity and benefit and means to reduce its toxicity.
• Understand how to carry out cryoprotectant toxicity testing in the development of preservation protocols
Cryopreservation of Human Pluripotent Stem Cells.
Dr. Charles Hunt, Independent, UK
Learning objectives:
• The special challenges of stem cell cultures for effective preservation
• The important features that need to be checked to assure recovery of a functional stem cell culture
• Procedures that may need to be implemented to assure the safety of stem cells for clinical use.
