Altogen Biosystems [ Ссылка ] provides the PC-3 Transfection Reagent for prostate cancer cells [ Ссылка ]. The PC-3 cell line originates from the bone marrow metastasis of a 62-year-old Caucasian man with grade IV prostate cancer post-mortem. The patient was treated with androgen suppression therapy. This cell line has a high metastatic potential and research indicates that the cells will form tumors in nude mice. The PC-3 cell line expresses Prostate Specific Antigen and has a low amount of testosterone-5-alpha reductase activity. This cell line has unique applications for research related to cancer and molecular and cellular biology due to its high metastatic potential. The PC-3 cell line is also considered to be a classic cell line in the study of prostate cancer.
-Altogen Biosystems offers the PC-3 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
The PC-3 Transfection Reagent is an advanced formulation of a lipid-based reagent, and it has been developed to provide high transfection efficiency with the PC-3 cell line.
This cell line is considered to be a classic cell line for studying prostate cancer. When cultured in vitro, PC-3 cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase PC-3 Transfection Kit at [ Ссылка ]
-PC-3 Transfection protocol:
1. Plate 10,000 - 15,000 PC-3 cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for 15 - 30 minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however, it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with PC-3 cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression 48 - 72 hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent 12-24 hours after transfection.
If the viability of PC-3 cells being transfected is affected at 16 - 24 hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant.
-General Lipoplex-mediated Transfection Mechanism of Action.
-PC-3 Transfection Kit Product details:
Cationic lipids formulation
Optimized for intracellular delivery of plasmid DNA, siRNA, microRNA, and mRNA
High transfection efficiency of both siRNA and plasmid DNA without compromising cell viability
Achieve robust siRNA uptake for dependable gene silencing
Effective transfection under conditions of up to 40% serum
Transfection kit includes Transfection Enhancer reagent
Gentle enough to be used for single cell analysis
Developed and manufactured by Altogen Biosystems ([ Ссылка ])
-Data demonstrates over 80% mRNA knockdown.
-Protein expression of GAPDH in PC-3 cells.
-PC-3 Transfection kit benefits:
Pre-optimized transfection protocol for PC-3 cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use PC-3 transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable PC-3 cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
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