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Formaldehyde-assisted Isolation of Regulatory Elements to Measure Chromatin Accessibility in Mammalian Cells - a 2 minute Preview of the Experimental Protocol

Alfonso Rodríguez-Gil, Tabea Riedlinger, Olesja Ritter, Vera V. Saul, M. Lienhard Schmitz
University Hospital Virgen del Rocío, Department of Oncohematology and Genetics. Institute of Biomedicine of Seville (IBiS); Justus-Liebig-University, Institute of Biochemistry, Medical Faculty, Friedrichstrasse 24, Member of the German Center for Lung Research;

The plasticity of nuclear architecture is controlled by dynamic epigenetic mechanisms including non-coding RNAs, DNA methylation, nucleosome repositioning as well as histone composition and modification. Here we describe a Formaldehyde-Assisted Isolation of Regulatory Elements (FAIRE) protocol which allows the determination of chromatin accessibility in a reproducible, inexpensive, and straightforward manner.

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