Welcome to our series describing basic mounting techniques for SEM examination of some common categories of specimens. In this video, we demonstrate a method for mounting samples using conductive silver liquid.
This mounting method is useful for applications where it is essential to keep the sample absolutely physically immobile, such as focused ion beam work, electron backscatter diffraction analysis (EBSD), atomic force microscopy (AFM), and high-resolution imaging at high tilt angles. It can also be used to mount samples with irregular shapes that do not adhere well to double-sided tape style adhesives. The silver liquid acts as a glue that maintains conductivity while fixing the sample to the SEM stub with no physical drift once fully dried.
Note:
The procedures described in this series are a good starting point for samples of this type, using consumables and equipment that can be obtained at the UNSW Electron Microscope Unit. Refinements may need to be made depending on the nature of the sample, configuration of the SEM, your specific analytical needs, and/or the materials available in your own facility.
Always follow health and safety protocols as described in your material's safety data sheet (SDS).
For more tailored advice, consult staff at the UNSW EMU, your node of Microscopy Australia, or the electron microscopy facility at your own institution.
Work Health and Safety Note:
Conductive silver liquid presents a range of potential hazards that include physiological effects (due to exposure through inhalation, ingestion, and/or skin contact) and environmental contamination.
To control these risks, this SEM mounting procedure includes: use of gloves, all handling conducted in a ducted fume cupboard with the sash pulled down low, and use of only small volumes of conductive silver liquid (less than 0.5 ml) from a dispenser of restricted size (~15ml). Any excess or residual conductive silver liquid is left to dry fully prior to disposal.
As with all work with a hazardous substance, always ensure you have read and understood the relevant SDS, Risk Management Form and Safe Work Procedure, and have all necessary controls in place prior to handling.
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Online Microscopy Training & SEM Simulator: [ Ссылка ]
Video by Charlie Kong & Karen Privat
