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Optimization of a Quantitative Micro-neutralization Assay - a 2 minute Preview of the Experimental Protocol
Yipu Lin, Yan Gu, John W. McCauley
The Francis Crick Institute, Mill Hill Laboratory;
This study describes an imaging-based micro-neutralization assay to analyze the antigenic relationships between viruses. The protocol employs a flatbed scanner and has four steps, including titration, titration quantitation, neutralization, and neutralization quantitation. The assay works well with current circulating influenza A(H1N1)pdm09, A(H3N2), and B viruses.
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