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Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting - a 2 minute Preview of the Experimental Protocol
Zerina Lokmic, Elizabeth S. Ng, Matthew Burton, Edouard G. Stanley, Anthony J. Penington, Andrew G. Elefanty
The Royal Children’s Hospital, Murdoch Childrens Research Institute; The University of Melbourne, Department of Paediatrics, Faculty of Medicine, Dentistry and Health Sciences; Monash University, Clayton, Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences;
The goal of this protocol is to isolate lymphatic endothelial cells lining human lymphatic malformation cyst-like vessels and foreskins using fluorescence-activated cell sorting (FACS). Subsequent cell culturing and expansion of these cells permits a new level of experimental sophistication for genetic, proteomic, functional and cell differentiation studies.
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