Historical Context
Scientific Curiosity: The Human Genome Project (HGP) was initiated in the 1990s, spurred by the curiosity to understand the genetic basis of life and diseases.
Discovery of DNA Structure: The 1953 discovery of DNA's double-helix structure by James Watson and Francis Crick laid the foundation for molecular genetics and set the stage for the HGP.
Goals of the HGP
Sequencing Human DNA: The primary goal was to sequence all 3 billion base pairs of human DNA, which make up the genome.
Gene Identification: The project aimed to identify and locate approximately 20,000-25,000 human genes, each carrying instructions for making proteins.
Functional Understanding: Researchers sought to understand the functions of these genes and their role in health and disease.
Comparative Genomics: The project also aimed to compare the human genome with those of other species to understand evolutionary relationships.
Sequencing Methods
Sanger Sequencing: This method, developed by Frederick Sanger, was used initially for sequencing DNA. It involved creating complementary DNA strands to determine the sequence of bases.
Next-Generation Sequencing (NGS): NGS revolutionized genomics by allowing parallel sequencing of millions of DNA fragments. It dramatically increased speed and lowered costs.
Bioinformatics and Data Analysis
Data Challenges: Genomic sequencing generates vast amounts of data, requiring specialized tools for storage and analysis.
Genome Assembly: The process of piecing together short DNA sequences (reads) into longer sequences (contigs) to reconstruct the full genome.
Gene Annotation: Identifying and labeling genes, regulatory regions, and other functional elements within the genome.

For Queries: mailtobioresource@gmail.com
Twitter:Me@BioResource

Related Videos:
---------------------------------------------------------------------------------------------------------------
Plotting Bacterial Growth Curve: [ Ссылка ]
Colony Forming Units: [ Ссылка ]
Serial Dilution Methods: [ Ссылка ]
Copy Number Calculation for qPCR: [ Ссылка ]
How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR: [ Ссылка ]
How to generate qPCR standard curve in excel and calculate PCR efficiency: [ Ссылка ]
Real Time qPCR optimization, Calculating PCR Efficiency: [ Ссылка ]
Taqman Assay Vs SYBR Green Assay: [ Ссылка ]
Resolving poor PCR efficiency: [ Ссылка ]
Calculating molecular weight of Unknown protein [ Ссылка ]
Designing qPCR Primers
[ Ссылка ]
Oligonucleotide Preparation & Purification [ Ссылка ]
How to check Oligo Concentration
[ Ссылка ]

свернуть