Technical Video Protocol for Megazyme's Mushroom and Yeast Beta-Glucan Assay (K-YBGL).
A procedure for the specific measurement and analysis of 1,3:1,6-β-glucan and 1,4:1,6-α-glucan in mushroom and mycelial products, yeast and fungal preparations.
00:05 Introduction
00:52 Principle
02:55 Reagent Preparation
05:31 Measurement of Total Glucan
05:35 (i) Solubilisation & Partial Hydrolysis
09:23 (ii) Measurement of Alpha and Beta-Glucan plus D-glucose in sucrose and free D-glucose
11:58 Measurement of Alpha-Glucan
12:03 (i) Solubilisation & Partial Hydrolysis of Alpha-Glucan
15:24 (ii) Measure of Alpha-Glucan, D-glucose in sucrose and free D-glucose
17:26 Calculations
19:48 Megazyme’s Publication
** Subtitles are available, but must be manually turned on and can be changed to your desired language **
This video will talk you through the assay principle, procedure, and will also explain how to perform the necessary calculations.
• Product page at the Megazyme webstore:
[ Ссылка ]
• Download the K-YBGL data booklet:
[ Ссылка ]
• Access the Mega-Calc for K-YBGL:
[ Ссылка ]
