This course is an introduction to Next Generation Sequencing for the study of RNA expression. Topics include:
✅ quantitative and qualitative analysis of RNA in a sample,
✅ data preparation using Next Generation Sequencing,
✅ preparation of a table of expression from raw FASTq files,
✅ Visualization of high-dimensional data using Principal Component Analysis (PCA).
✅ Mapping raw reads to reference genome and transcriptome
✅ Detecting junctions and assembly of isoforms
✅ Quantification of mRNA: Gene, isoform and exon expression table

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or register for the next round of our live program dedicated to RNA-Se data analysis by visting the following link: [ Ссылка ]

Video Chapters:
0:00 Introduction
0:56 NEXT GENERATION SEQUENCING DATA
1:38 Omics: Next Generation Sequencing (NGS)
3:34 Next Generation Sequencing (NGS) Data
3:53 Data Formats for Sequence Data
4:28 Mapping reads: Genes and isoforms
5:17 RNA-Seq: Sequencing Data
5:47 RNA-Seq: overview
6:51 Expression Table (FPKM or TPM)
7:08 Transcriptomics Course Collection

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