This video describes step-by-step how to make a custom miniature incubator / chamber for an upright automated biological/industrial microscope. The chamber allows control of temperature in the base, lid and objective lens, humidity and CO2/O2 and most importantly scanning a big area of a sample such as one or two 35mm petri-dishes, chamber slides and so on, in automated mode while the chamber is fully closed. The video talks about some challenges during the development in terms of how to fit this chamber to the microscope and meet all requirements of having a closed chamber for motorized stage.
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