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In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration - a 2 minute Preview of the Experimental Protocol
Mark E. Kusek, Michael A. Pazos, Waheed Pirzai, Bryan P. Hurley
Harvard Medical School, Department of Pediatrics; MGH for Children, Department of Pediatric Gastroenterology; Massachusetts General Hospital, Mucosal Immunology and Biology Research Center;
Neutrophil trans-epithelial migration in response to mucosal bacterial infection contributes to epithelial injury and clinical disease. An in vitro model has been developed that combines pathogen, human neutrophils, and polarized human epithelial cell layers grown on transwell filters to facilitate investigations towards unraveling the molecular mechanisms orchestrating this phenomenon.
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