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Measurement of Force-Sensitive Protein Dynamics in Living Cells Using a Combination of Fluorescent Techniques - a 2 minute Preview of the Experimental Protocol
Katheryn E. Rothenberg, Ishaan Puranam, Brenton D. Hoffman
Duke University, Department of Biomedical Engineering;
Here, we present a protocol for the simultaneous use of Förster resonance energy transfer-based tension sensors to measure protein load and fluorescence recovery after photobleaching to measure protein dynamics enabling the measurement of force-sensitive protein dynamics within living cells.
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