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An Efficient Method for Adenovirus Production - a 2 minute Preview of the Experimental Protocol
Madalina Dumitrescu, Violeta Georgeta Trusca, Ioana Madalina Fenyo, Anca Violeta Gafencu
Institute of Cellular Biology and Pathology "Nicolae Simionescu",;
Here, we present a protocol for adenovirus production using the pAdEasy system. The technology includes the recombination of the pAdTrack and pAdEasy-1 plasmids, the adenovirus packaging and amplification, the purification of the adenoviral particles from cell lysate and culture medium, the viral titration, and the functional testing of the adenovirus.
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