A new single molecule approach to study DNA repair protein dynamics:
seeing is believing
Speaker: Ben van Houten, University of Pittsburgh Cancer Institute, USA
Chair: David Rueda, Imperial College, London/MRC-LMS, UK
Co-Chair: Christopher Batters, MRC Laboratory of Molecular Biology, UK
Ben van Houten describes a new single molecule approach observing DNA binding proteins from nuclear extracts of cells expressing fluorescently tagged proteins. He lists the advantages of this workflow to look at order of assembly, effects of mutation, post-translation modification. In addition, cell extracts may contain protein chaperones which improve assembly. Using this method he investigated the binding of PARP1, a protein involved in DNA damage repair, to lambda DNA attached to optically trapped beads in a LUMICKS C-trap. He could delineate the binding kinetics and hence the affinity of the interaction and then looks at the effect of mutations and drug binding on this interaction. Lastly, he examined the binding of PARP1 binding to nucleosomes.
Read more about Ben van Houten’s work here:
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You can find more about the Next Generation Biophysics Symposium 2024 here:
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About the MRC Laboratory of Molecular Biology (LMB):
The LMB is one of the world's leading research institutes. Discoveries and inventions developed at the LMB, for example DNA sequencing and methods to determine the structure of proteins, have revolutionised all areas of biology. Our scientists work to advance understanding of biological processes at the molecular level. This information will help us to understand the workings of complex systems, such as the immune system and the brain, and solve key problems in human health.
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