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Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses - a 2 minute Preview of the Experimental Protocol

Fengwei Zhang, Yanan Wang, Xuechai Shang, Shanshan Wang, Rong Xiao, Hongjuan Zhou, Long Cai
Hang Zhou Red Cross Hospital, Central Laboratory; Yuhang District Maternity and Child Health Care Hospital, Clinical Laboratory;

This protocol describes an experimental process to produce high-titer infectious viral pseudotyped particles (pp) with envelope glycoproteins from two influenza A strains and how to determine their infectivity. This protocol is highly adaptable to develop pps of any other type of enveloped viruses with different envelope glycoproteins.

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