When you’re busy in the lab, you don’t always have time to make new LB/agar plates containing every antibiotic you need. Luckily, there is a relatively quick way to add antibiotic to your plates in a pinch. Watch this video to learn how to perform over-agar antibiotic plating.
For the full written Over-Agar Antibiotic Plating protocol, visit:
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0:00 Intro
0:57 Materials
1:24 Preparation Before Plating
2:24 Plating
3:50 Incubation & Observation
4:25 Setting Up a Control Plate
4:46 Adding Multiple Antibiotics and Conclusion
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