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Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells - a 2 minute Preview of the Experimental Protocol
Yonatan R. Lewis-Israeli, Brett D. Volmert, Mitchell A. Gabalski, Amanda R. Huang, Aitor Aguirre
Michigan State University, Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology; Michigan State University, Department of Biomedical Engineering, College of Engineering;
Here, we describe a protocol to create developmentally relevant human heart organoids (hHOs) efficiently using human pluripotent stem cells by self-organization. The protocol relies on the sequential activation of developmental cues and produces highly complex, functionally relevant human heart tissues.
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