MicroScale Thermophoresis (MST) is based on the directed movement of molecules in a temperature gradient which strongly depends on a variety of molecular properties such as size, charge, hydratation shell or conformation thus being a powerfull technique to quantify biomolecular interactions.

Application examples:

Protein folding
Thermodynamics
Interactions in complex liquids
KD determination
Enzyme kinetics

We operate with the Nano Temper technologies Monolith NT.115 (BLUE/RED) that measures biomolecular interactions via detection of fluorescent dyes or fluorescent fusion proteins, providing a broad application range to detect interactions between any type of biomolecules and for pM to mM binding affinities. The MO.Affinity Analysis software allows for a straightforward analysis and evaluation of the MicroScale Thermophoresis data. Binding parameters such as dissociation constants (Kd) or EC50 values and can be easily quantified and compared.

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