Altogen Biosystems [ Ссылка ] provides the ASMC Transfection Reagent for aortic smooth muscle cells [ Ссылка ]. The ASMC or Aortic Smooth Muscle Cells originate from the human tunica intima and tunica media of healthy aorta tissue. The tunica media is made up of mostly smooth muscle cells as well as elastic fibers that are arranged in spirals; it is the middle layer of the artery. The tunica intima is the inner layer of the artery and it is made up of a layer of elastic fibers and a network of connective tissue. ASMC cells can synthesize glycosaminoglycan, myosin, collagen and elastin and can also differentiate in response to various factors. The ASMC cells can be used to study atherosclerosis, angiogenesis, stroke, diabetes, vascular biology, diabetes and other human vascular disorders. ASMC cells are sensitive to IGF1 and IGF2, but resistant to insulin at the receptor level. Via the inhibition of MMP-9 expression, curcumin can prevent migration of ASMC cells.
-Altogen Biosystems offers the ASMC Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
ASMC Transfection Reagent is an advanced formulation of a lipid-based reagent, and it has been developed to provide high transfection efficiency with ASMC and other vascular cell lines.
This cell line is a good host for studying human vascular disease. When cultured in vitro, ASMC cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase ASMC Transfection Kit at [ Ссылка ]
-ASMC Transfection protocol:
1. Plate 10,000 - 15,000 ASMC cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for 15 - 30 minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with ASMC cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression 48 - 72 hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent 12-24 hours after transfection.
If the viability of ASMC cells being transfected is affected at 16 - 24 hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant.
-General Lipoplex-mediated Transfection Mechanism of Action.
-ASMC Transfection Kit Product details:
Two component formulation enhances lipid mediated transfection efficiency
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocol
High transfection efficacy in the presence of serum
Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
Reproducible transfection results
Works well for standard reverse transfection and high-throughput applications
Developed and manufactured by Altogen Biosystems ([ Ссылка ])
-Data demonstrates over 80% mRNA knockdown.
-Protein expression of GAPDH in ASMC cells.
-ASMC Transfection kit benefits:
Pre-optimized transfection protocol for ASMC cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use ASMC transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable ASMC cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
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