Presented By: Bon-Kyoung Koo, PhD
Speaker Biography: Dr. Bon-Kyoung Koo is an experienced mouse geneticist actively employing the emerging organoid culture technology to study stem cell maintenance and activation in the mouse and human intestine and colon. Following his PhD in the laboratory of Prof. Young-Yun Kong at POSTECH, Dr. Koo joined the group of Prof. Hans Clevers at the Hubrecht Institute, Netherlands in 2009.
Webinar: CRISPR-Cas assisted genetics in intestinal organoids
Webinar Abstract: The identification of LGR5 + intestinal stem cells helped us to understand various aspects of adult stem cells and led to the establishment of primary 3D intestinal organoid culture system from mouse and human tissues. This novel culture system faithfully recapitulates various aspects of the intestinal epithelium in vitro with remarkable long-term expansion capacity and genetic stability. Thus, the model is recognized as a suitable in vitro model system for genetic studies. To exploit all the potential of this culture, protocols have been fully optimized for primary establishment, maintenance, cryopreservation, plasmid transfection and viral transduction. A number of examples will be shown to introduce how to apply CRISPR technology and organoid models for genetic studies, including simultaneous paralogue knockout, functional genetic screening and precise gene correction.
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