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Generation, Purification, and Characterization of Cell-invasive DISC1 Protein Species - a 2 minute Preview of the Experimental Protocol

Verian Bader, Philipp Ottis, Martin Pum, Joseph P. Huston, Carsten Korth
Medical School Düsseldorf, Germany, Department of Neuropathology; University of Düsseldorf, Center of Behavioral Neuroscience;

The generation, purification and cell invasion of intracellular, cytoplasmic full length DISC1 protein aggresomes from cell cultures and of a labeled, multimeric recombinant DISC1 protein fragment in E. coli are described. Cell invasiveness is shown for recipient cells in cell culture and for neurons in vivo after stereotactical brain inoculation.

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