The analytical toolbox for the analysis of carbohydrates as well as carbohydrate-based materials is not a full as those for materials such as proteins, nucleic acids, and lipids. Considering the fact that carbohydrates are the most abundant materials on the planet, the inability to rapidly isolate, characterize and/or synthesize glycomaterials hinders our understanding of basic biochemical processes. Hyphenated techniques that couple a separation process to mass spectrometry are central to resolving key issues that impact all facets of life. Several examples of on-going LCMS-based research projects ranging from the determination of sialic acid levels toglycopeptide analysis will be presented, highlighting the importance of improving our understanding of the role of carbohydrates in core processes.

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