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Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition - a 2 minute Preview of the Experimental Protocol

Sidharth Tyagi, Donald Beqollari, Chang Seok Lee, Lori A. Walker, Roger A. Bannister
University of Colorado School of Medicine, Department of Medicine-Cardiology Division; Baylor College of Medicine, Department of Molecular Physiology and Biophysics;

Immunohistochemical staining of myosin heavy chain isoforms has emerged as the state-of-the-art discriminator of skeletal muscle fiber-type (i.e., type I, type IIA, type IIX, type IIB). Here, we present a staining protocol along with a novel semi-automated algorithm that facilitates rapid assessment of fiber-type and fiber morphology.

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