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Preparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates - a 2 minute Preview of the Experimental Protocol
Kayla F. Goliwas, Lindsay M. Miller, Lauren E. Marshall, Joel L. Berry, Andra R. Frost
University of Alabama at Birmingham, Department of Pathology; University of Alabama at Birmingham, Department of Biomedical Engineering;
We demonstrate a method to generate 3D breast cancer surrogates, which can be cultured using a perfusion bioreactor system to deliver oxygen and nutrients. Following growth, surrogates are fixed and processed to paraffin for evaluation of parameters of interest. The evaluation of one such parameter, cell density, is explained.
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