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Detection of Trypanosoma brucei Variant Surface Glycoprotein Switching by Magnetic Activated Cell Sorting and Flow Cytometry - a 2 minute Preview of the Experimental Protocol
Danae Schulz, Monica R. Mugnier, Catherine E. Boothroyd, F. Nina Papavasiliou
Rockefeller University, Laboratory of Lymphocyte Biology; Harvey Mudd College, Department of Biology; Masters School,;
African trypanosomes grown in vitro undergo antigenic variation at a low rate, such that populations are made up of parasites expressing a dominant variant surface glycoprotein (VSG) type and a small population of "switched" variants. This protocol describes a fast method for detecting and quantifying these populations.
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