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Novus Biologicals Visual Protocols: In phase 1 of the western blot procedure, you will learn how to prepare your samples before loading them into a gel. Here we isolate protein from cultured cells, quantify total protein concentrations with a BCA assay, add loading buffer to the sample, and heat the sample. Additional help can be found in the support section of [ Ссылка ], through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.
