This video explains How recombinant insulin is produced?
00:00 Introduction
00:45| Why Humulin or recombinant insulin is a milestone in
rDNA technology?
What were the previous insulin sources?
Advantages of recombinant insulin
02:15 | Discovery of Structure of Insulin by Frederick Sanger
02:50| 6 Steps in rDNA technology
03:43 | Step 1: Identification & Isolation of Insulin gene
04:25 |Step 2: Insertion of this isolated gene in a suitable expression vector
vector used for recombinant insulin production
06:30| Step 3: Introduction of this vector into a suitable host; E.coli
Hosts used for recombinant insulin production
07:42| Step 4: Selection of the transformed host cell with insulin gene
08:45| Step 5: Expression of insulin gene in the host
09:42| Step 6: Purification of insulin protein
What is recombinant fusion protein? and its uses
11:30| Summary of Steps Involved in the production of Recombinant Insulin
▶Steps in recombinant DNA technology [ Ссылка ]
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