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Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling - a 2 minute Preview of the Experimental Protocol

Mame Daro Faye, Tyson E Graber, Martin Holcik
University of Ottawa, Apoptosis Research Centre, Children's Hospital of Eastern Ontario Research Institute and Department of Biochemistry, Microbiology and Immunology; Montreal Neurological Institute,; University of Ottawa, Apoptosis Research Centre, Children's Hospital of Eastern Ontario Research Institute and Department of Pediatrics;

The ability of cells to adapt to stress is crucial for their survival. Regulation of mRNA translation is one such adaptation strategy, providing for rapid regulation of the proteome. Here, we provide a standardized polysome profiling protocol to identify specific mRNAs that are selectively translated under stress conditions.

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