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Construction and Use of an Electrical Stimulation Chamber for Enhancing Osteogenic Differentiation in Mesenchymal Stem/Stromal Cells In Vitro - a 2 minute Preview of the Experimental Protocol

Liudmila Leppik, Mit B. Bhavsar, Karla M.C. Oliveira, Maria Eischen-Loges, Sahba Mobini, John H. Barker
Johann Wolfgang Goethe-University, Frankfurt Initiative for Regenerative Medicine; University of Florida, J. Crayton Pruitt Family Department of Biomedical Engineering;

Here we present a protocol for the construction of a cell culture chamber designed to expose cells to various types of electrical stimulation, and its use in treating mesenchymal stem cells to enhance osteogenic differentiation.

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