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RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera) - a 2 minute Preview of the Experimental Protocol

Ying Wang, Nicholas Baker, Gro V. Amdam
Arizona State University , School of Life Sciences; Norwegian University of Life Sciences, Department of Chemistry, Biotechnology, and Food Science;

In this protocol, we describe two strategies that simultaneously suppress two genes (double gene knockdown) in honey bees. Then we present how to use the proboscis extension response (PER) assay to study the effect of double gene knockdown on honey bee gustatory perception.

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