Join us for a chat with Bio-Rad application scientists about the current issues most relevant to your research. Jump to specific topics by following the links below.

In this episode, we discuss: Mixed Mode Chromatography Tips
- CHT column packing tips at process scale
- Overview of multimodal interactions with biomolecules
- Purification strategies for mAbs and viruses

We had so many great audience questions. Watch the whole video or quickly jump to a specific question:
00:10 Introduction
3:10 What defines a resin as a mixed-mode resin?
6:10 Is there a generic strategy you can recommend for screening and optimizing your method with a mixed-mode resin?
7:30 How can we improve protein purification, especially from a crude extract from animal tissue?
10:38 Considering there are multiple modes of interactions, how can we pick the right resin for the protein of interest, especially in research when we do not know a lot about the protein other than theoretical PI, MW, etc? We usually have less knowledge about hydrophobicity.
15:57 Could you do empty-full AAV virus separation using mixed-mode resin?
17:39 I have a 5-step purification process. What do you recommend to reduce the number of steps?
20:20 Has CHT been used in virus or viral vector purification?
21:15 Do you recommend certain mixed-mode resins for capture step or polishing steps?
23:40 Considering uncertainties come with mixed mode, can we consider them as the first line of selection or after traditional ones?
27:12 Some people have promoted the use of CHT in a calcium mode, up to 10 mM calcium in the buffer. What are your thoughts on this variation of running CHT? Also, other eluents such as sulfate and borate have been proposed. Any comments?
29:55 What are the different modifiers apart from salt that can be added to elute proteins in mixed mode?
33:10 How does CHT differ from other mixed modes?
39:02 When I am scaling up, are there any recommendations for bed height/compression factor? Flow rate?
43:18 What are the scaling up recommendations for all your mixed-mode resins?
45:18 For acid-sensitive MAbs, what resin is a good solution in the capture step from cell supernatant? Is buffer exchanging required?
46:23 I did not get optimal selectivity from a mixed-mode resin, do you have recommended protocols for running DOE?
47:59 Would you recommend CHT for mRNA purification?
49:50 What are the recommendations for sanitizing a CHT column?
52:38 Are there Bio-Rad mixed-mode resins used in FDA approved products?
53:28 Where can I get more information about mixed-mode resins? What are the best resources/papers?
55:25 Conclusion


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