How to collect fluorescence intensity measurements with the Tecan Spark multimode microplate reader and SparkControl Software. Topics covered include:
click on time stamps to jump to specific topics
Plate selection @0:46
Selecting settings for Flashes @7:34
Wavelengths and Bandwidth, and following the Minimum Distance Rule @3:05
Gain @8:20
Z position Optimization @12:52
Multiple Reads Per Well @15:16
Taking a Reading @16:30
Changing settings to influence signal intensity and background @19:10

This video is not official Tecan content and it is not a substitute for official training by a Tecan employee. The information contained in this video is for educational purposes only and is not comprehensive. Your data quality and accuracy is not guaranteed by virtue of following the instructions in this video. Your results may vary.

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