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MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria - a 2 minute Preview of the Experimental Protocol
Noémie Mercier, Karine Prévost, Eric Massé, Pascale Romby, Isabelle Caldelari, David Lalaouna
Université de Strasbourg, CNRS,; Université de Sherbrooke, Department of Biochemistry;
MAPS technology has been developed to scrutinize the targetome of a specific regulatory RNA in vivo. The sRNA of interest is tagged with a MS2 aptamer enabling the co-purification of its RNA partners and their identification by RNA sequencing. This modified protocol is particularly suited for Gram-positive bacteria.
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