Watch the Full Video at [ Ссылка ].
Isolation and Functional Characterization of Human Ventricular Cardiomyocytes from Fresh Surgical Samples - a 2 minute Preview of the Experimental Protocol
Raffaele Coppini, Cecila Ferrantini, Alessandro Aiazzi, Luca Mazzoni, Laura Sartiani, Alessandro Mugelli, Corrado Poggesi, Elisabetta Cerbai
University of Florence, Department NeuroFarBa, Division of Pharmacology; University of Florence, Department of Clinical and Experimental Medicine, Division of Physiology;
Current knowledge on the cellular basis of cardiac diseases mostly relies on studies on animal models. Here we describe and validate a novel method to obtain single viable cardiomyocytes from small surgical samples of human ventricular myocardium. Human ventricular myocytes can be used for electrophysiological studies and drug testing.
Visit [ Ссылка ] to explore our entire library of 14,000+ videos of laboratory methods and science concepts.
JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching.
Subscribe to our channel: [ Ссылка ]
