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Culturing Caenorhabditis elegans in Axenic Liquid Media and Creation of Transgenic Worms by Microparticle Bombardment - a 2 minute Preview of the Experimental Protocol
Tamika K. Samuel, Jason W. Sinclair, Katherine L. Pinter, Iqbal Hamza
University of Maryland, Department of Animal and Avian Sciences; University of Maryland, Department of Cell Biology and Molecular Genetics;
C. elegans is usually grown on solid agar plates or in liquid cultures seeded with E. coli. To prevent bacterial byproducts from confounding toxicological and nutritional studies, we utilized an axenic liquid medium, CeHR, to grow and synchronize a large number of worms for a range of downstream applications.
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