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Three-dimensional Co-culture Model for Tumor-stromal Interaction - a 2 minute Preview of the Experimental Protocol

Masafumi Horie, Akira Saito, Yoko Yamaguchi, Mitsuhiro Ohshima, Takahide Nagase
The University of Tokyo, Department of Respiratory Medicine, Graduate School of Medicine; The University of Tokyo, Department of Clinical Laboratory, Graduate School of Medicine; The University of Tokyo, Division for Health Service Promotion; Nihon University School of Dentistry, Department of Biochemistry; Ohu University School of Pharmaceutical Sciences, Department of Biochemistry;

Here we present a protocol to co-culture in three-dimensions, which is useful for investigating multicellular interactions and extracellular matrix-dependent modulation of cancer cell behavior. In this experimental model, cancer cells are cultured on collagen gels embedded with human cancer-associated fibroblasts.

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