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RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells - a 2 minute Preview of the Experimental Protocol
Nadejda Sigal, Anna Pasechnek, Anat A. Herskovits
Tel Aviv University, The Department of Molecular Microbiology and Biotechnology, The George S. Wise Faculty of Life Sciences;
Here we describe a method for bacterial RNA isolation from Listeria monocytogenes bacteria growing inside murine macrophages. This technique can be used with other intracellular pathogens and mammalian host cells.
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