Human embryonic kidney (HEK) cells are one of the most commonly used cell models used in preclinical and cell/molecular biology research and are known for their high transfection efficiency. HEK-293 is a cell line that was, in 1977, first established from sheared adenovirus 5 DNA-transformed HEK cells. This transformation resulted in ~4.5kb of viral genome being incorporated into human chromosome 19 of the human embryonic kidney cells. The HEK-293 cell model is so widely used that it is often referred to as a “cellular test tube” for pharmaceutical, investigative and biochemical purposes. HEK-293 cells have also been used for therapeutic protein and gene therapy virus production. Altogen Biosystems’ lipid-based HEK-293 specific pre-optimized transfection kit has over 90% efficiency that has been qRT-PCR verified:
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