Northern Blot is a crucial technique to detect specific RNA molecules in a sample. In the first step, RNA molecules from the sample are separated on a gel using Gel Electrophoresis. Next, the separated RNA fragments are transferred to a Nylon Membrane. This transfer involves a special arrangement.
This video explains:
1. Northern Blot
2. Northern Blotting technique (with animation)
3. Applications of Northern Blotting
First, the Agarose gel is placed on a sponge, which is dipped in a buffer. Now, the Nylon membrane is placed on the gel. Next. we place the filter paper, a glass plate and weight above the Nylon membrane. For further details. WATCH THE VIDEO...
