Altogen Biosystems [ Ссылка ] provides the Caki-1 Transfection Reagent for kidney epithelial cells [ Ссылка ]. The Caki-1 cell line originated from the renal carcinoma cells of a 49-year old male Caucasian patient with clear cell carcinoma. The Caki-1 cell line is tumorigenic and also displays epithelial morphology. The cell line has implications for studying human proximal tubule epithelium. The Caki-1 cell line contains few filaments, but several microvilli. Interestingly, the Caki-1 cell line has an extensive Golgi apparatus and endoplasmic reticulum as well as several small mitochondria.
-Altogen Biosystems offers the Caki-1 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
Caki-1 Transfection Reagent is an advanced cationic lipid formulation based reagent, and it has been developed to provide high transfection efficiency with Caki-1 cells.
This cell line is a good host for studying human proximal tubule epithelium. When cultured in vitro, Caki-1 cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase Caki-1 Transfection Kit at [ Ссылка ]
-Caki-1 Transfection protocol:
1. Plate 10,000 - 15,000 Caki-1 cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for 15 - 30 minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however, it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with Caki-1 cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression 48 - 72 hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent 12-24 hours after transfection.
If the viability of Caki-1 cells being transfected is affected at 16 - 24 hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant.
-General Lipoplex-mediated Transfection Mechanism of Action.
-Caki-1 Transfection Kit Product details:
Two component formulation enhances lipid mediated transfection efficiency
Optimized easy-to-use transfection protocol provided for transfection of siRNA, DNA, mRNA, and microRNA
Kit includes Transfection Enhancer reagent and recommended transfection protocol
High transfection efficacy in the presence of serum
Expand your RNAi application with a reagent optimized for delivery of both siRNA and plasmid
Reproducible transfection results
Works well for standard reverse transfection and high-throughput applications
Developed and manufactured by Altogen Biosystems ([ Ссылка ])
-Data demonstrates over 80% mRNA knockdown.
-Protein expression of GAPDH in Caki-1 cells.
-Caki-1 Transfection kit benefits:
Pre-optimized transfection protocol for Caki-1 cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use Caki-1 transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable Caki-1 cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
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