This movie was created for the publication: 'Clathrin coat controls synaptic vesicle acidification by blocking vacuolar ATPase activity'. It depicts a model of the formation of the clathrin coated neuronal vesicle. The three participating protein types are: vATPase [in the center], AP2 [with 2 long arms] and clathrin [the cage proteins, light brown]. The aim of the animation was to show how the clathrin cage building might lead to a blockade of the vATPase activity. This might happen when one clathrin arm dislocates the central rod of the vATpase. the animation can be broken down in single steps. 1. AP2 binds to clathrin and leads it to the vATPase 2. Formation of the clathrin coat around the vATPase - inactivation 3. During cage building, the vesicle is formed 4. vesicle moves inward 5. clatrhin coat leaves vesicle, vATPase active again
