Historic DNA from museum or herbarium specimens is highly fragmented. Generating NGS sequence libraries using customized non-commercial protocols (non-kit) is very time-consuming and thus not suitable for high throughput. We describe a simple kit-based protocol in our video using the NEBNext® Ultra™ II DNA Library Prep Kit.
For more information about the protocol:
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00:00 Introduction
03:55 Step 1 - NEBNext End Prep
05:48 Step 2 - Adaptor Ligation
08:21 Step 3 - Cleanup of Adaptor-ligated DNA without Size Selection
12:49 Step 4 - PCR Enrichment of Adaptor-ligated DNA
15:45 Step 5 - Cleanup of PCR Reaction
20:36 Step 6 - DNA Sequencing
21:40 Credits
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