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Isolation of CD4+ T-cells and Analysis of Circulating T-follicular Helper (cTfh) Cell Subsets from Peripheral Blood Using 6-color Flow Cytometry - a 2 minute Preview of the Experimental Protocol
Elliot Byford, Matthew Carr, Lucia Piñon, Matthew J. Ahearne, Simon D. Wagner
University of Leicester, Leicester Cancer Research Centre and Ernest and Helen Scott Haematology Research Institute; University of Leicester, MRC Toxicology Unit;
Here, a protocol for the isolation and characterization of CD4+ T-cell subsets from human peripheral blood is described. Purified CD4+ T-cells are analyzed by flow cytometry to determine proportions of T-follicular helper cell subsets.
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