Olympus FluoView FV3000 Confocal Microscope workflow at Sydney Microscopy and Microanalysis
Equipped with 405, 488, 561 and 640 nm lasers and a transmitted light detector allowing for the majority of fluorophores to be imaged with a DIC (or brightfield) overlay. With the fastest Resonance Scanner on the market, it can capture up to 438 fps at 512 x 32 or 30 fps at 512 x 512. This, combined with a stage top incubator results in excellent temporal resolution for imaging live cells.
0:00 - Start up
3:36 - Sample loading
4:57 - Confocal imaging (single slice)
7:33 - Saving an image
8:05 - Confocal imaging (z-stack)
10:01 - Shut down
Sydney Microscopy and Microanalysis
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These videos provide guidance only. Local work health and safety protocols should always take precedence when you undertake any lab procedures in your own facility. Some of the videos show workflows for specific instruments and their content should not be taken as specific instructions for other types of instruments.
