This tutorial demonstrates how use Protein BLAST to align and compare two or more amino acid sequences. To initiate an alignment, in this demonstration, a reference protein sequence is entered into the query box and compared to additional protein sequences that are entered into the subject box. Using the descriptions tab of the alignment results, it is possible to get an idea of the coverage as well as the percent identity between each subject alignment with the query. Assessing the alignments using pairwise with dots for identities allows for a quick visualization of differences between two sequences including single amino acid substitutions as well as deletions and insertions.
Protein BLAST: [ Ссылка ]
Genomic Education at the Jackson Laboratory: [ Ссылка ]
BLAST Tutorial Series Playlist: [ Ссылка ]
Key Moments:
0:00 Introduction
0:39 Navigating to Protein BLAST (BLASTp)
0:56 Comparing two or more DNA sequences
1:23 What is a reference sequence?
1:29 Entering a Query sequence
2:04 Entering Subject sequences
2:45 BLAST results page navigation
3:12 What is coverage?
3:39 What is percent identity?
4:11 Visualizing sequence alignments
5:36 Interpreting amino acid changes
Keywords: BLAST, Protein BLAST, protein sequence comparison, gene variants
